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81.
82.
Correlations between morphological and genetic data provide evidence to delineate species or evolutionarily significant units, which then become the units to conserve in management plans. Here, we examine the distribution and genetic differentiation of two morphotypes of short‐finned pilot whale (Globicephala macrorhynchus) in the Pacific Ocean. Mitochondrial control region sequences from 333 samples were combined with 152 previously published sequences to describe genetic variability globally and population structure in the Pacific. Although genetic variability is low, we found strong differentiation at both broad and local levels across the Pacific. Based on genetics, two types are distributed throughout the Pacific, one predominantly in the eastern Pacific and the other in the western and central Pacific. In the eastern Pacific Ocean, no correlation was found between distribution and sea surface temperature. The two types have broad latitudinal ranges, suggesting their distributions are likely driven by more complex factors, such as prey distribution, rather than sea surface temperature.  相似文献   
83.
不同施肥处理对甜菊生长及糖苷含量和积累量的影响   总被引:1,自引:0,他引:1  
采用盆栽法,以甜菊( Stevia rebaudiana Bertoni)品种‘中山4号'(‘Zhongshan No.4')当年生扦插苗为研究对象,研究不同形态氮肥(硫酸铵、硝酸钠和尿素)及不同施氮量(纯氮)、施磷量( P2 O5)和施钾量( K2 O)对幼苗生长及糖苷含量和单株积累量的影响。结果显示:随氮肥、磷肥和钾肥施用量的提高,甜菊幼苗的株高、茎粗、叶长、叶宽、单株叶干质量和单株茎干质量均呈先升后降的变化趋势,且总体上与对照无显著差异,仅施磷量300 mg·kg-1处理组的叶长显著高于对照;根据施肥量与单株叶干质量的回归方程,确定硫酸铵、硝酸钠、尿素、磷肥和钾肥的施用量分别为64.87、660.21、735.84、211.54和775.92 mg·kg-1时,幼苗单株叶干质量最高。在硫酸铵处理组中,300 mg·kg-1处理组甜菊叶片中的莱鲍迪苷A( R-A)含量及甜菊苷( St)、R-A和总苷的单株积累量以及600 mg·kg-1处理组的St单株积累量高于对照,多数处理组的St、R-A和总苷含量及单株积累量均低于对照;在硝酸钠处理组中,1200 mg·kg-1处理组的R-A和总苷含量、600和900 mg·kg-1处理组的St单株积累量以及300~900 mg·kg-1处理组的R-A和总苷单株积累量高于对照,其他处理组的St、R-A和总苷含量及单株积累量均低于对照;在尿素处理组中,1500 mg·kg-1处理组的R-A和总苷的含量和单株积累量以及600和900 mg·kg-1处理组的R-A和总苷单株积累量高于对照,其他处理组的St、R-A和总苷含量及单株积累量均低于对照;各施氮处理组中,仅1500 mg·kg-1处理组的R-A含量与对照差异显著,其他指标均与对照无显著差异。在施磷处理组中,100 mg·kg-1处理组的R-A含量以及100和200 mg·kg-1处理组的St、R-A和总苷的单株积累量高于对照,多数处理组的St、R-A和总苷含量及单株积累量低于对照且与对照均无显著差异。在施钾处理组中,各处理组的St、R-A和总苷含量及单株积累量均高于对照,其中仅900 mg·kg-1处理组的St、R-A和总苷含量与对照显著差异。各施肥处理组的St含量占总苷含量的百分率均低于对照、R-A含量占总苷含量的百分率均高于对照,且总体上与对照无显著差异。经过综合分析,建议在甜菊生育期内的施肥量为纯氮600~900 mg·kg-1、P2 O5200~300 mg·kg-1和K2 O 600~900 mg·kg-1,其中氮肥以尿素为宜。  相似文献   
84.
描述了鸢尾科鸢尾属宽柱鸢尾(Iris latistyla Y.T.Zhao)的1个新变型——白花宽柱鸢尾(I.latistyla Y.T.Zhao f.albifloraJ.Luo)。原变型的花被为蓝紫色,而新变型花被为白色。并对其潜在的药用研究价值进行了讨论。  相似文献   
85.
In this study, we compared the chemical forms and subcellular distribution of Cd in high-Cd (X16) and low-Cd (N88) sweet potato cultivars through hydroponic experiments and examined the Cd distribution in their roots by histochemical staining. The results showed that inorganic and pectate/protein-integrated Cd predominated in the leaves, and Cd concentrations were significantly higher in X16 than in N88. However, in the roots, Cd was mostly integrated with pectate and protein, and Cd concentration was higher in N88 than in X16. It was mainly stored through vacuolar sequestration and cell wall binding. In the leaves and stems, Cd concentrations in all subcellular fractions were higher in X16 than in N88; the opposite was observed in the roots. In X16, Cd was mostly accumulated in the root stele, and its Cd translocation factor was higher than that of N88. Overall, the subcellular fractions of X16 roots retained less Cd than N88 roots, and more Cd entered the root stele of X16 and subsequently moved to the shoots. The higher amounts of inorganic, water-soluble, and pectate/protein-integrated Cd with high mobility in the shoots of X16 than in N88 might facilitate Cd remobilization to other tissues, but this needs to be further studied.  相似文献   
86.
A spectrofluorimetric method for the determination of eptifibatide is presented based on its native fluorescence. The type of solvent and the wavelength of maximum excitation and emission were carefully selected to optimize the experimental conditions. Under the specified experimental conditions, the linearities obtained between the emission intensity and the corresponding concentrations of eptifibatide were in the range 0.1–2.5 μg/ml for the calibration curve constructed for direct determination of eptifibatide in dosage form and 0.05–2.2 μg/ml for the calibration curve constructed in spiked human plasma with a good correlation coefficient (r > 0.99). The lower limit of quantification for the calibration curve constructed in human plasma was 0.05 μg/ml. Recovery results for eptifibatide in spiked plasma samples and in dosage form, represented as mean ± % RSD, were 95.17 ± 1.94 and 100.29 ± 1.33 respectively. The suggested procedures were validated according to the International Conference on Harmonization (ICH) guidelines for the direct determination of eptifibatide in its pure form and dosage form and United States Food and Drug Administration (US FDA) Guidance for Industry, Bioanalytical Method Validation for the assay of eptifibatide in human plasma.  相似文献   
87.
Aims The nutrient uptake, requirement and releasing rates of bryophytes are very different from those of tracheophytes. However, it is difficult to make a quantitative evaluation of bryophytes’ roles in nutrient cycling and their specific eco-physiological adaptations due to lack of knowledge of their concentrations and stoichiometric ratios of carbon (C), nitrogen (N) and phosphorus (P). To fill this gap, the present study aims to investigate: (i) what are the elevational trends of C, N and P concentrations and stoichiometric ratios of bryophytes? (ii) whether C, N and P concentrations and stoichiometric ratios of bryophytes differ between different bryophyte types (in terms of the growth form and living substrate)? and (iii) how do the exponent scalings of N and P of bryophytes change along the elevational gradient?  相似文献   
88.
Previous studies have shown that switchgrass has a wide range of genetic variation and that productivity is linked to local adaptation to the location of origin for many cultivars. In this meta‐analysis, we compiled and analyzed 900 observations associated with 41 field trials for four switchgrass cultivars (two lowlands, Alamo and Kanlow, and two uplands, Cave‐In‐Rock and Shelter). This extensive dataset and machine learning were used to identify the most influential variables impacting switchgrass productivity, to search for evidence of local adaptation to each cultivar's location of origin, and to predict change in productivity under future climate for each cultivar. In general, variables associated with climate and management are more important predictors of productivity relative to soil variables. Three climatic variables, annual mean temperature, annual precipitation, and precipitation in the wettest month, are identified as key environmental variables for productivity of all cultivars. Productivity under future climate (2041–2060) is predicted to stay stable for all cultivars relative to the prediction under current climate (1986–2005) across all trial locations and over a 20‐year simulation period. However, the productivity of each cultivar varies from location to location and from year to year, although productivity varies more between locations than between years. Additionally, we observe shifts in the most productive cultivar at the local field scale depending on the combination of management practice and climates. The shape of the relationship between productivity and the annual mean temperature relative to the cultivar's location of origin is a bell‐shaped curve for Kanlow, Cave‐in‐Rock, and Shelter, indicative of local adaptation. Identifying influential environmental variables and their relationships to productivity with respect to cultivar's location of origin help predicting productivity on the local field scale, and will help with the biofuel production planning through the selection of suitable cultivars for different locations under climate changes.  相似文献   
89.
90.
Previously we have reported on a series of pyridine-3-carboxamide inhibitors of DNA gyrase and DNA topoisomerase IV that were designed using a computational de novo design approach and which showed promising antibacterial properties. Herein we describe the synthesis of additional examples from this series aimed specifically at DNA gyrase, along with crystal structures confirming the predicted mode of binding and in vitro ADME data which describe the drug-likeness of these compounds.  相似文献   
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